Why is an EDTA tube used for CBC?

edta tubes tests

Samples from kidney transplant patients were placed in BD Vacutainer glass EDTA tubes. EDTA acts as an anticoagulant, binds the calcium ions and stops the blood sample from clotting. Blood collection tubes that contain heparin, which stabilizes red blood cell membranes, are used for specialized hematological studies, such as. B. for red blood cell fragility testing and several specialized chemical tests. Blood collection tubes that contain heparin, which stabilizes red blood cell membranes, are used for specialized hematological studies, such as. B. for red blood cell fragility testing and several specialized chemical tests.

Many laboratories have carried out their own assessments of plastic tubes to document analyte compatibility before conversion but have not always published their results. Blood collection tubes that contain heparin, which stabilizes red blood cell membranes, are used for specialized hematological studies, such as. B. for red blood cell fragility testing and several specialized chemical tests. EDTA is used for most hematological procedures to determine the complete blood count, prepare EDTA plasma, and collect whole blood and bone marrow samples.

What does the EDTA tube test for?

The moment you break through the endothelial lining of your patient’s blood vessel to collect a sample, this cascade of events is initiated and may continue within the blood tube once removed from the body. Hold the tube horizontally over the slide, release the pressure of your finger from the end, and tilt the tube slightly vertically to allow a controlled amount of blood to flow from the tube onto the slide. When comparing serum volume in the tube, they observed a significant reduction in phenytoin, phenobarbital, carbamazepine, quinidine and lidocaine concentrations after storage of 500 μl of serum compared with 1 ml in glass and plastic gel tubes. Coagulated blood is obtained in blood collection tubes that contain a silicone coating or a serum separator.

Why is EDTA used in blood collection?

The use of EDTA to measure cytokines, proteins and peptides, as well as heart markers, is described, giving an overview of the protection of unstable molecules by this anticoagulant. It is not only used to prevent blood from clumping but also to treat extreme cases of lead poisoning in a process known as chelation therapy. The defense simply had to prove that the blood from the tube was the same blood that was found in Halbach’s car. The most important advantage of EDTA is that it doesn’t distort blood cells, making it ideal for most hematology tests.

Why is the EDTA tube used for CBC?

To ensure accurate test results, all tubes containing an anticoagulant or preservative must be completely filled. Well-prepared films can be used to measure the differential white blood cell count, study the morphology (size, structure and shape) of red and white cells, determine the presence of abnormal cells, and also study the size and number of platelets. If multiple tests are ordered that require frozen serum, a plastic transport tube should be prepared for each test. Blood collected with an anticoagulant appropriate for the test described cannot be considered suitable for other tests.

What test is performed in the EDTA tube?

We calculated the ISI for EDTA samples, and no clinically significant difference was found between citrate and EDTA INR results. The regression equation for INR from citrate and EDTA samples depended on the reagent used, not just the sample dilution or the anticoagulant.. Using the regression equation (INR or sec) to analyze INR results from EDTA samples is clinically acceptable and allows EDTA samples to be used for PT measurement with citrate calibrators. Always use the correct vacuum tube for tests that require a special anticoagulant (e.g.. B. EDTA, heparin, sodium citrate, etc.) or a preservative is required.

The ISI determination for EDTA samples requires a mathematical calculation of EDTA ISI as in the present study or EDTA-based ISI calibrators.

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References:

Gabriel Nieves

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