Centrifuge- Hemolysis can be caused by rough handling of a blood sample, by allowing the tourniquet to run too long (causing blood congestion), or by squeezing the fingertip too much during capillary collection, dilution, exposure to contaminants, extreme temperatures, or pathological conditions. Blood cells are suspended in plasma, which consists of water and dissolved materials, including hormones, antibodies, and enzymes that are transported to tissues, as well as cellular waste products that are transported to the lungs and kidneys. Send plasma to a plastic transport tube marked “Plasma, Sodium Heparin” or “Plasma, Lithium Heparin.” If multiple samples are required, remove the first collection tube from its holder as soon as blood flow is interrupted, turn the first tube upside down to prevent clotting, and gently insert the second tube into the holder.
Do you rotate serum tubes?
After proper centrifugation, the serum may be left in contact with the gel barrier of the SST tubes for up to 5 days if stored properly. Serum or plasma should be separated from cells within 2 hours of collection to avoid erroneous test results. The serum can be sent into the centrifuge tube with an intact barrier (correct separation during centrifugation) between cells and serum, or in a plastic transport tube. If serum is not immediately analyzed, serum should be divided into 0.5 ml aliquots, stored, and transported at —20 °C or less.
After centrifugation, it is important to immediately transfer the liquid component (serum) into a clean polypropylene tube using a Pasteur pipette.