What is the correct order of tubes for a blood draw?

Blood draw- Only blood culture tubes, non-additive glass serum tubes, or plastic serum tubes without clot activator may be collected in front of the coagulation tube. Blood samples must be taken by phlebotomists in a specific order to avoid cross-contamination of the sample with additives in different collection tubes. Special considerations are included for collections of vascular access devices, blood culture samples, collections in isolated environments, and for dealing with emergency situations. The order of the phlebotomy is the same for samples taken with a syringe, tube holder, or in tubes that were pre-evacuated at the time of collection.


order of the phlebotomy is the same for samples taken with a syringe, tube holder, or in tubes that were pre-evacuated at the time of collection. To avoid cross-contamination, blood must be drawn and collected in tubes in a specific order.

Which color tube is used for blood sampling?

If a Coag tube (light blue) is the only or first tube to be drawn, a 5 ml disposal tube must be drawn first.

How does a phlebotomist know which tube to use?

This is because when a doctor orders a blood test, what they want to test (laboratory use) is on the request form, NOT IN THE COLOR OF THE TUBE. The order of drawing is a sequence of tubes that phlebotomists should follow to avoid testing complications and cross-contamination of additives Each color has a unique additive that either a clot activator (helps with blood clotting) or an anticoagulant (prevents blood coagulation) is. This may seem obvious, but when students try to study the tubes, they can easily be overwhelmed by the amount of information.

When the patient is positioned so that the tubes are tilted upright relative to a horizontal plane, they fill from bottom to top. In addition, the tube may contain a substance called thixotropic gel, which helps separate the blood layers.

Which pipes are always blood drawn first in a standard sequence?

Evidence of the need for a specific order in which blood collection tubes should be filled was first published over 30 years ago, but the concept remains elusive for many healthcare professionals with sampling tasks. It’s a great way to remember the Order of drawing (OOD) because you can change the sentence so that it’s most helpful to you. As a licensed phlebotomist, it’s important not only to know the order of blood collection but also to know the correct procedure and standard for taking blood. This standard recommends drawing EDTA tubes first to ensure good sample quality, followed by other additive tubes, and finally serum sample tubes


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