In the average adult male, there are approximately 5 quarts (4.75 liters) of blood, consisting of approximately 3 quarts (2.85 liters) of plasma and 2 quarts (1.9 liters) of cells. When performed, it allows the technologist to microscopically view the actual appearance of red and white blood cells. If multiple samples are required, remove the first collection tube from its holder as soon as blood flow is interrupted, turn the first tube upside down to prevent clotting, and gently insert the second tube into the holder. Most laboratory tests are performed using serum, anticoagulated plasma, or whole blood.
How many ml are EDTA tubes?
Permissible complete blood count values of underfilled K (EDTA tubes) in powder form can be determined with just 1.0 ml of blood. Yes, BD Vacutainer K2EDTA Plus tubes are intended for routine immunohematology testing, including red blood cell grouping, Rh typing, and antibody screening. Yes, the BD Vacutainer PPT Tube (reference number 36278) contains an EDTA anticoagulant and an inert polyester gel. BD Vacutainer spray-coated EDTA tubes are used for hematologic whole blood testing, immunohematology testing, and donor screenings.
Current laboratory standards from the Clinical Laboratory Standards Institute (CLSI) and manufacturers (Becton Dickinson) suggest that underfilling K (EDTA) blood collection tubes may result in incorrect hematological values.
How much blood is in an EDTA tube?
The venipuncture should be minimally traumatic to minimize platelet activation and should be performed with a needle of at least 23 g (21-22 g is ideal for small animals and 18-20 g is ideal for large animals) and a 3-5 ml syringe (depending on the desired volume). Blood smears should be taken at the time of collection to minimize storage-related changes that always occur. Push the slide quickly along the entire length of the slide and draw a thin line of blood behind you. Immediately after removing the needle from the vein, gently touch the tip of the needle with one of the clean slides. This results in a small drop of blood with a diameter of about 1 to 2 mm, about the size of a match head.
What size is a Vacutainer?
One-inch needles are used for routine venipuncture, and 1 ½ inch needles are used for patients with very deep veins. For blood type typing, HLA phenotyping, DNA and paternity testing, BD offers a wide range of tubes to meet your specific testing requirements.