How long must a sample clot for before a serum sample can be centrifuged?

serum sample

Serum sample- After taking whole blood, allow the blood to coagulate by leaving it undisturbed at room temperature. After centrifugation, it is important to immediately transfer the liquid component (plasma) into a clean polypropylene tube using a Pasteur pipette. If serum is not immediately analyzed, serum should be divided into 0.5 ml aliquots, stored, and transported at —20 °C or less. After centrifugation, it is important to immediately transfer the liquid component (serum) into a clean polypropylene tube using a Pasteur pipette.

Heparinized tubes (green tips) are indicated for some applications. However, heparin can often be contaminated with endotoxin, which can stimulate white blood cells to release cytokines.

How long should a serum sample tube be taken before centrifugation?

To transport swab samples or tissue, remove the cap, place the sample in the tube (break off the swab handle if necessary) and put the cap back on, and pull If in doubt or question about the type of swab to be removed If the sample is passed, it is imperative to call the laboratory to clarify the sequence and sample requirements. When centrifuging an individual or multiple tubes, each tube must be balanced with a blood tube or a tube filled with water so that it fits the tube directly opposite the centrifuge head. If sample requirements require a chilled sample (2-8 °C), the sample should be placed immediately in a small plastic tube bag, tied, and placed in crushed ice or a mixture of ice and water.

How long should the serum curdle?

If frozen serum is required, place the plastic transport tubes (prepared above) immediately in the freezer compartment of the refrigerator. Remove the stopper and carefully suck all serum out of the cells, using a separate disposable pipette for each tube. If multiple tests are ordered that require frozen serum, a plastic transport tube should be prepared for each test. The serum is obtained from coagulated blood that has not been mixed with an anticoagulant (a chemical that prevents blood from clotting).

In addition, the gel barrier may not be intact and may result in improper separation of serum and cells, potentially affecting test results. The effect of serum clot contact time on laboratory results was investigated by dividing each blood sample into four blood collection tubes.

When can you centrifuge a serum sample?

Samples from test subjects with different stages of cancer or other diseases, as well as from people without the disease, are important tools in the search for biomarkers, predictors, or tests that can be used to identify serious diseases earlier or more easily than is currently possible. The Early Detection Research Network (EDRN) Standard Operating Procedure (SOP) for collection of EDTA plasma and serum. A sample taken from a blood collection tube with a clot activator should be inverted five times to facilitate the coagulation process.

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